TY  - GEN
T1  - Resolving a contradiction: full gene deletion of kqt-3 by CRISPR does not lead to pharyngeal pumping defects
AU  - Bauer, Rosemary
AU  - Nebenfuehr, Benjamin
AU  - Golden, Andy
DO  - 10.17912/micropub.biology.000137
UR  - http://beta.micropublication.org/journals/biology/micropub-biology-000137/
AB  - KQT-3 is the C. elegans ortholog of human KCNQ1, a voltage-gated potassium channel that is implicated in multiple types of Long Q-T cardiac arrhythmias including Jervell and Lange-Nielsen syndrome and Romano-Ward syndrome. In order to assess the possibility of modeling these syndromes in C. elegans, we obtained the tm542 deletion allele, which had previously been associated with changes to the defecation cycle (Nehrke et al., 2008, Kwan et al. 2008). Any visible, easily-scored phenotype would be an indication that we could move forward with creating patient-specific missense alleles by CRISPR.
PY  - 2019
JO  - microPublication Biology
ER  -