TY  - GEN
T1  - New mutants defective in RMED/V neuron specification are alleles of EOR-1 and EOR-2
AU  - Huang, Xun
AU  - Jin, Yishi
DO  - 10.17912/micropub.biology.000139
UR  - http://beta.micropublication.org/journals/biology/micropub-biology-000139/
AB  - In a genetic screen for genes affecting RMED/V neuron specification, we isolated two mutants, ju190 and ju198 (Huang et al., 2002; Huang et al., 2004; Huang and Jin, 2019). We mapped ju190 to the X chromosome, a region covered by three cosmids (H01A20, C44H4 and F54E4), between unc-9 and unc-3, using the snip-SNP mapping strategy. The novel conserved nuclear transcription factor eor-2 is contained in the cosmid C44H4, and eor-2(cs42) mutant animals exhibit similar behavior defects as ju190 (Rocheleau et al., 2002). We introduced Punc-25GFP into eor-2(cs42), a null allele, and found no expression in RMED/V cell, as for ju190 (Huang and Jin, 2019). DNA sequencing analysis of the eor-2 genomic DNA from homozygous ju190 animals identified a C to T nucleotide transition that results in an Opal stop at Arg721, in the conserved C-terminal domain (Figure 1A). Therefore, the RMED/V defects in ju190 arise from a complete loss of EOR-2 function.
PY  - 2019
JO  - microPublication Biology
ER  -