TY  - GEN
T1  - Tools for Mos1-mediated single copy insertion (mosSCI) with excisable unc-119(+) or NeoR (G418) selection cassettes
AU  - Aram, Reta
AU  - MacGillivray, Kailynn
AU  - Li, Chengyin
AU  - Saltzman, Arneet L
DO  - 10.17912/micropub.biology.000146
UR  - http://beta.micropublication.org/journals/biology/micropub-biology-000146/
AB  - Insertion of single-copy transgenes is an important tool in C. elegans genome engineering. Following the discovery that the Drosophila mariner element Mos1 could mobilize in the C. elegans germline (Bessereau et al. 2001), tools for efficient Mos1-mediated transgene insertion have been developed and widely adopted (Robert and Bessereau 2010). In parallel, the establishment of effective antibiotic selection in C. elegans offered increased versatility for genetic crosses and the advantage of injecting into phenotypically wild-type animals (Giordano-Santini et al. 2010, Semple et al. 2010).
PY  - 2019
JO  - microPublication Biology
ER  -