TY  - GEN
T1  - Temperature-dependent mitochondrial-nuclear epistasis
AU  - Cazares-Navarro, Enrique
AU  - Ross, Joseph A
DO  - 10.17912/micropub.biology.000147
UR  - http://beta.micropublication.org/journals/biology/micropub-biology-000147/
AB  - The mitochondrial and nuclear genomes contain genes encoding the protein subunits comprising mitochondrial electron transport chain complexes I–V (Blanchard and Lynch 2000). This dynamic has established a co-evolutionary relationship between the two genomes: when a deleterious mutation occurs in one genome, a compensatory mutation in the other genome might restore efficient oxidative phosphorylation (Blier et al. 2001). The epistatic interactions between different co-evolved mito-nuclear alleles can then be disrupted when mating between two genetically diverse populations occurs (Rand et al. 2004). Such hybridization introduces non-compensatory nuclear alleles that can again disrupt efficient electron transport (Burton et al. 2013). The nematode Caenorhabditis briggsae exhibits such evidence of mito-nuclear epistasis, where experimental recombination of the nuclear genome from the wild isolate AF16 with the mitochondrial genome of wild isolate HK104 (and vice versa) resulted in hybrid dysfunction (Chang et al. 2016). Further, AF16-HK104 advanced-intercross recombinant inbred lines (AI-RIL) show strong epistatic interactions between the mitochondrial and nuclear genomes, particularly with the X chromosome (Haddad et al. 2018). AF16 and HK104 are representatives of the genetically divergent tropical and temperate clades of C. briggsae (Cutter et al. 2006). Because of this phylogeographic population structure and knowing that mitochondrial genetic variation can play important roles in environmental adaptation (Das 2006, Camus et al. 2017, Lamb et al. 2018), we tested the hypothesis that mitochondrial-nuclear epistatic interactions in C. briggsae are temperature-dependent. We hybridized AF16 with a different temperate population, HK105. We created ten (10) replicate AF16-HK105 AI-RIL for both (2) cross directions at both (2) 20°C and 25°C. We then genotyped these forty (10x2x2) RIL at a single locus on the X chromosome to measure segregation distortion and its dependence on cross direction (and thus on cytotype and mitotype).
PY  - 2019
JO  - microPublication Biology
ER  -