TY  - GEN
T1  - Double mutants of TTLL glutamylase genes have little to no difference in viability or brood size
AU  - Shah, Pooja
AU  - Martinez, Monica
AU  - Chakraborty, Monica
AU  - Kota, Sharwani
AU  - Shah, Bhumi
AU  - Lee, Jessica
AU  - Peel, Nina
DO  - 10.17912/micropub.biology.000156
UR  - http://beta.micropublication.org/journals/biology/micropub-biology-000156/
AB  - The tubulin tyrosine ligase like (TTLL) family of enzymes is required for microtubule glutamylation (Janke et al., 2005). Disruption of microtubule glutamylation has been associated with impaired centriole integrity, cilia degeneration, and aberrant neuronal function (Bobinnec et al., 1998; Gadadhar et al., 2017). C. elegans has five glutamylating TTLL enzymes (Chawla et al., 2016). Deletion of individual TTLL enzymes does not impair viability or brood size, moreover microtubule morphology in the early embryo is unaffected (Chawla et al., 2016). To test for potential redundancy between TTLL enzymes we constructed all combinations of double TTLL mutants. Double mutant genotypes were confirmed by PCR. Embryonic viability (percentage of embryos hatching), and brood size (the total number of embryos produced per worm) were determined over the entire reproductive lifespan for a minimum of 10 worms for each genotype, and compared with N2 worms as a wild type control. All experiments were carried out at 20°C.
PY  - 2019
JO  - microPublication Biology
ER  -