TY  - GEN
T1  - The GLR-1 phenotypes of the daf-7(e1372) allele are not temperature sensitive
AU  - McGehee, Annette
DO  - 10.17912/micropub.biology.000158
UR  - http://beta.micropublication.org/journals/biology/micropub-biology-000158/
AB  - The DAF-7/TGF-β signaling pathway plays a developmental role in the dauer decision; mutants in this signaling pathway have temperature-sensitive constitutive dauer phenotypes (Golden and Riddle 1984, Thomaset al. 1993).  Additionally, the DAF-7/TGF-β signaling pathway acts in later developmental stages as an environmental sensor to regulate several aspects of metabolism and behavior in response to environmental cues (Greer et al. 2008, Guimenny and Savage-Dunn 2013, Meisel et al. 2014, Fletcher and Kim 2017). Recently it was shown that the DAF-7/TGF-β signaling pathway is required for normal regulation of levels of the glutamate receptor GLR-1 (McGehee et al. 2015).  The effect of the DAF-7/TGF-β signaling pathway on GLR-1 was determined both directly using GFP-tagged GLR-1 protein (GLR-1::GFP) and indirectly by measuring the frequency of spontaneous reversals, a behavior that is controlled by signaling through GLR-1 (Zheng et al. 1999).  The daf-7(e1372) allele is described as a temperature-sensitive allele (Riddle et al. 1997).  When this strain is grown at 15°C worms progress normally through development, and when the growth temperature is shifted to 25°C worms enter the dauer stage (Golden and Riddle 1984).  Experiments investigating the effect of the DAF-7/TGF-β signaling pathway on GLR-1 were conducted by growing animals at 15°C, followed by growth at 25°C for several hours prior to performing experiments (McGehee et al. 2015).  This method of growing the worms at a low temperature and then shifting to a higher temperature is frequently used in studies using DAF-7/TGF-β signaling pathway mutants.  However, some of the phenotypes associated with these mutants are not temperature sensitive, for example egg laying (Trent et al. 1983), decreased pumping rate and increased fat deposition (Greer et al. 2008). These observations suggest that it is the dauer phenotype that is temperature sensitive and not necessarily the daf-7(e1372) allele.
PY  - 2019
JO  - microPublication Biology
ER  -