TY  - GEN
T1  - Using HDR and a template to introduce an in-frame HA tag on the 3′ end of the Xenopus laevis gata2.L open reading frame
AU  - Piccinni, Maya
AU  - Sharpe, Colin
AU  - Guille, Matt
DO  - 10.17912/micropub.biology.000170
UR  - http://beta.micropublication.org/journals/biology/micropub-biology-000170/
AB  - Protein detection is a powerful technique used broadly in research, however the successful use of antibodies has its practical limitations presented by the lack of knowledge about the protein in question, poor accessibility of the target sites, specificity barrier and cross-reactivity with other proteins (Fritze and Anderson, 2000). In these cases the insertion of a known short amino acid sequence into the desired locus can be considered. This sequence allows the protein in question to be traceable and detectable by a pre-existing commercial well-characterized antibody. Since the first ever tag was developed in 1943 (Rawson, 1943) there have been many scientific and technological advances which allowed the development of the clustered, regularly interspaced, short palindromic repeats (CRISPR) technology which we use here to insert an HA tag (YPYDVPDYA) into the 3’ end of the Xenopus laevisgata2.Lgene (NM_001090574.1).
PY  - 2019
JO  - microPublication Biology
ER  -