TY  - GEN
T1  - Expression of the DELLA repressor GAI and its regulators SPY and SEC are impacted by disruption of chromatin modifiers
AU  - Trachtman, Natalie
AU  - Sockler, Patrick
AU  - Caiola, Hanna
AU  - McCain, Elizabeth R
AU  - Hark, Amy T
DO  - 10.17912/micropub.biology.000175
UR  - http://beta.micropublication.org/journals/biology/micropub-biology-000175/
AB  - Since trichome number is altered with disruption of GCN5 or ADA2b (Kotak et al. 2019; Wang et al. 2019), we sought to determine whether these chromatin factors might impact the expression of known regulators of trichome initiation, including two members of the DELLA family of repressor proteins, GA-Insensitive (GAI) and REPRESSOR of ga1-3 (RGA).  As their names suggest, these proteins are sensitive to gibberellin (GA) signaling (Silverstone et al. 2001).  GA complexed with its receptor can bind to DELLA proteins and promote their interaction with a component of an E3 ubiquitin ligase, SLEEPY1 (SLY1), leading to subsequent proteasome-media degradation (Dill et al. 2004).  In addition to SLY1, we also assessed the expression of SPINDLY (SPY) and SECRET AGENT (SEC), both known to covalently modify RGA and impact its functionality in a positive and negative manner respectively (Zentella et al. 2016, 2017).
PY  - 2019
JO  - microPublication Biology
ER  -