TY  - GEN
T1  - Caenorhabditis elegans strain sensitivity to sodium arsenite exposure is varied based on age and outcome measured
AU  - Hershberger, Kathleen A
AU  - Leuthner, Tess C
AU  - Waters, Tanner A
AU  - Meyer, Joel N
DO  - 10.17912/micropub.biology.000186
UR  - http://beta.micropublication.org/journals/biology/micropub-biology-000186/
AB  - Arsenic is a toxicant with multiple intracellular targets, including many mitochondrial enzymes. Previous work in C. elegans has shown that strains deficient in complexes I, II, and III (but not complex V) have increased sensitivity to sodium arsenite, suggesting that the electron transport chain is a key target of arsenic toxicity (Luz et al. 2016). However, in that work, the only outcome used to determine strain sensitivity to arsenic was larval growth. Based on these results, we used complex mutants (nuo-6 (complex I), mev-1 (complex II), isp-1 (complex III), and atp-2 (complex V)) to further explore the physiological effects of sodium arsenite induced electron transport chain dysfunction. We used three different assays to ask the same question: which electron transport chain mutant strain is most sensitive to sodium arsenite? Interestingly, we found that strain sensitivity to sodium arsenite depends on the outcome measured. These data indicate that single assays may not provide a complete picture of nematode response to an exposure and suggests that complete characterization of nematode response requires analyzing multiple endpoints.
PY  - 2019
JO  - microPublication Biology
ER  -