TY  - GEN
T1  - Nibbling 405 kb off the X: Viable deletion alleles eliminating 50 protein coding genes, including a chromatin factor involved in neuronal development
AU  - Minevich, Gregory
AU  - Bernstein, Alex
AU  - Mei, Kevin
AU  - Poole, Richard J
AU  - Hobert, Oliver
DO  - 10.17912/micropub.biology.000187
UR  - http://beta.micropublication.org/journals/biology/micropub-biology-000187/
AB  - We are interested in isolating mutants that affect lineage specification in the nervous system of the nematode C. elegans. The harsh touch sensory neuron PVD, generated by the postdeirid lineage, can be labeled with two transgenically expressed fluorophores, ser-2::gfp (otIs138 transgene)(Tsalik et al., 2003) and dop-3:rfp (vsIs33 transgene)(Chase et al., 2004). Using a otIs138; vsIs33 double transgenic strain, we screened for EMS-induced mutations in which both markers fail to be expressed in PVD and isolated two strains in which the majority of animals fail to display reporter expression in PVD (ot642 mutant strain: 56% animals showed no marker expression in PVD expression; ot575 mutant strain: 71% of animals; n=54). ot575 and ot642 fail to complement each other. Animals that carry these alleles are viable, fertile and display no obvious morphological abnormalities. Both strains were subjected to Illumina whole genome re-sequencing, one in combination with Hawaiian SNP mapping (ot642)(Doitsidou et al., 2010) the other in combination with variant discovery mapping (ot575)(Minevich et al., 2012). These two orthogonal mapping approaches revealed that both mutant strains carry the exact same alteration: a loss of 405,058 bp from the extreme left end of the X chromosome (Figure 1). ot575 and ot642 were isolated from separate rounds of screens so these mutants were not progeny of the same initial parent. Each of these strains were isolated with the otIs138[ser2prom3::gfp] transgene that is also located on chromosome X so it is possible that this transgene somehow contributes to chromosome instability in some way or form.
PY  - 2019
JO  - microPublication Biology
ER  -