TY  - GEN
T1  - A panel of fluorophore-tagged daf-16 alleles
AU  - Aghayeva, Ulkar
AU  - Bhattacharya, Abhishek
AU  - Hobert, Oliver
DO  - 10.17912/micropub.biology.000210
UR  - http://beta.micropublication.org/journals/biology/micropub-biology-000210/
AB  - daf-16 encodes a broadly expressed transcription factor that functions in a wide variety of developmental and physiological processes (Lin et al., 1997; Ogg et al., 1997; Tissenbaum, 2018), including in the nervous system (Kim and Webb, 2017). DAF-16 protein shows highly dynamic cytoplasmic to nuclear translocation, which, in the past, has been visualized using multicopy constructs, which can produce potential overexpression artifacts (such as those described in (Henderson and Johnson, 2001)). To avoid such overexpression effects, the generation of fluorescently tagged daf-16 allele would be useful. Similarly, the generation of conditional alleles of daf-16 would be useful to resolve a number of open questions in regard to the focus of daf-16 action. To address both issues, we recently generated an mNeonGreen tagged daf-16 allele that also contained an auxin-inducible degron (Bhattacharya et al., 2019; Zhang et al., 2015). This allele, daf-16(ot853[daf-16::mNG::AID]), enabled us to provide a proof of concept for neuron-type specific daf-16 depletion (Bhattacharya et al., 2019). One issue with this allele has been that due to the emission spectrum of its fluorescent tag (mNeonGreen), it cannot be used in conjunction with gfp-based phenotypic read-outs.
PY  - 2020
JO  - microPublication Biology
ER  -