TY  - GEN
T1  - Auxin-independent depletion of degron-tagged proteins by TIR1
AU  - Schiksnis, Erin C
AU  - Nicholson, Angela L
AU  - Modena, Matthew S
AU  - Pule, Makena N
AU  - Arribere, Joshua A
AU  - Pasquinelli, Amy E
DO  - 10.17912/micropub.biology.000213
UR  - http://beta.micropublication.org/journals/biology/micropub-biology-000213/
AB  - The auxin inducible degron (AID) system was developed to enable conditional depletion of proteins of interest. In this system, a plant-derived F-box protein, TIR1, is expressed and complexes with endogenous Skp1 and Cullin proteins to form an E3 ubiquitin ligase complex. Proteins of interest can be tagged with a plant-derived degron, which is recognized by the TIR1-ubiquitin ligase complex in the presence of the plant hormone auxin (indole-3-acetic acid) (Nishimura et al. 2009). This system was recently adapted for Caenorhabditis elegans, wherein TIR1 constitutive expression enables rapid depletion of degron-tagged proteins of interest when animals are transferred to NGM plates supplemented with auxin. In C. elegans, the AID system has allowed for fine-tuned spatiotemporal control of protein expression and has been used to deplete many essential proteins, which are difficult to study using classical genetic approaches (Zhang et al. 2015). Here, we highlight a limitation of this system by demonstrating that TIR1 expression can cause depletion of degron-tagged proteins without auxin addition. These results corroborate observations from human cell lines, where TIR1 has been shown to act independently of auxin to chronically deplete degron-tagged proteins (Sathyan et al. 2019).
PY  - 2020
JO  - microPublication Biology
ER  -