TY  - GEN
T1  - Crossing two sperm chromatin-localized mCherry transgenes into a single C. elegans strain boosts signal intensity without harming sperm function
AU  - Wong, Amanda C
AU  - He, Jiajia
AU  - Wiltsie, Ashley R
AU  - Krawiec, Victoria S
AU  - Stanfield, Gillian M
DO  - 10.17912/micropub.biology.000214
UR  - http://beta.micropublication.org/journals/biology/micropub-biology-000214/
AB  - A formidable barrier to imaging studies of in vivo C. elegans sperm behavior has been the lack of bright, sperm-specific fluorescent markers. Use of dim sperm markers negatively influences the ability to perform confocal imaging in various ways, including but not limited to loss of signal deep into the gonad, which can be ~40 mm thick (Hubbard and Greenstein 2000); loss of spatial and temporal resolution, due to techniques commonly used to address imaging dim signals, such as signal accumulation; and increased phototoxicity and bleaching, due to increased excitation intensity. For in vivo imaging of the C. elegans hermaphrodite gonad, temporal resolution is limited by the rapidity of sheath contraction (McCarter et al. 1999) and the small physical size of sperm cells, parameters that already push the limits of many imaging systems. Researchers may find themselves trapped in the so-called “pyramid of frustration,” which refers to the difficult position of balancing the conflicting demands of high contrast, spatial resolution, temporal resolution, and sample health (Laissue et al. 2017).
PY  - 2020
JO  - microPublication Biology
ER  -