TY  - GEN
T1  - A simple PCR-based method to follow and genotype alleles with single nucleotide changes
AU  - Morin, Marie-Charlotte
AU  - Hoff-Yoessle, Sarah
AU  - Jarriault, Sophie
DO  - 10.17912/micropub.biology.000218
UR  - http://beta.micropublication.org/journals/biology/micropub-biology-000218/
AB  - Mutant analysis and the building of complex strains bearing several mutant alleles and/or transgenes are precious tools routinely used in studies using genetic models such as C. elegans. Crossing, strain building and genotyping necessitate to follow the various alleles to be combined during the crosses, some of which may not exhibit any easily detectable phenotype. Phenotypic markers (usually associated with an obvious phenotype: e.g. dpy, unc, rol, lon, etc) have been used as balancers in trans to follow a mutation of interest. This strategy is best used when the marker gene is located close enough to the mutation that one wishes to follow, to avoid crossing overs and recombinations during the several crosses and homozygosing steps. In recent years, using MosSCI insertions, Christian Frokjær-Jensen et al. have generated a set of strains bearing fluorescent markers inserted at specific chromosomal locations that can also be used as dominant genetic markers (Frokjaer-Jensen et al. 2014).
PY  - 2020
JO  - microPublication Biology
ER  -