TY  - GEN
T1  - Drosophila Plc21C is involved in calcium wave propagation during egg activation
AU  - Hu, Qinan
AU  - Vélez-Avilés, Adriana N.
AU  - Wolfner, Mariana F.
DO  - 10.17912/micropub.biology.000235
UR  - http://beta.micropublication.org/journals/biology/micropub-biology-000235/
AB  - Mature oocytes arrest in meiosis by the end of oogenesis and need to be activated in order to proceed to embryonic development. This egg activation process encompasses a series of events that transition the oocyte to a developing embryo, including meiosis resumption, maternal protein translation/modification, maternal mRNA processing, and cytoskeleton and eggshell changes (reviewed in Avilés-Pagán and Orr-Weaver, 2018; Horner and Wolfner, 2008a; Kashir et al., 2014; Krauchunas and Wolfner, 2013). The triggers of egg activation vary across species, from mechanical pressure in arthropods to the fertilizing sperm in nematodes, echinoderms and vertebrates (reviewed in Horner and Wolfner, 2008a). Despite differences in trigger, a rise of intracellular calcium levels preceding downstream events is found in most species studied to date (reviewed in Swann and Lai, 2016). In Drosophila egg activation, the intracellular calcium rise is triggered by mechanical cues, which can be pressure exerted by the oviduct or from oocyte swelling in vivo (Heifetz et al., 2001) or in vitro due to osmotic pressure from a hypotonic buffer (Horner and Wolfner, 2008b; Page and Orr-Weaver, 1997). This calcium rise takes the form of a transient calcium wave starting from the pole(s) and traversing the oocyte (Kaneuchi et al., 2015; York-Andersen et al., 2015). This calcium wave is initiated by influx of environmental calcium through Trpm channels in response to the mechanical trigger (Hu and Wolfner, 2019). Further propagation of the calcium wave requires release of internal calcium from stores through the inositol 1,4,5-trisphosphate (IP3) receptor (IP3R) calcium channel (Kaneuchi et al., 2015). It remains unclear how the initiation of calcium waves triggers the activation of IP3R during this process.
PY  - 2020
JO  - microPublication Biology
ER  -