TY  - GEN
T1  - The fasn-1(g14ts) allele is a Gly1830Arg missense mutation in C. elegans FASN-1
AU  - Bai, Xiaofei
AU  - Woodbury, David
AU  - Golden, Andy
DO  - 10.17912/micropub.biology.000244
UR  - http://beta.micropublication.org/journals/biology/micropub-biology-000244/
AB  - We had previously identified the causative mutation in emb-14(g43ts) as an Ala1425Thr missense mutation in the C. elegans fasn-1 gene on LGI (Jaramillo-Lambert et al. 2015). At that time, it was suggested that the emb-14 gene be renamed fasn-1. FASN-1 is a fatty acid synthase orthologous to human FASN (Lee et al. 2010). The g43ts allele is a tight, highly penetrant temperature-sensitive allele. There are presumably two other alleles, g14ts and fr8. We characterized the osmotic sensitivity of g14ts embryos, as well as the brood size and embryonic viability of the g14ts allele. We also demonstrated that g14ts failed to complement the tight temperature-sensitive allele, g43ts. We also determined the sequence alteration of g14ts to be a Gly1830Arg missense mutation upon sequencing PCR fragments that covered the entire fasn-1 gene (Fig. 1A). fr8 was previously identified as an M241I missense mutation in the fasn-1 gene. Interestingly, 40% of fasn-1(fr8) embryos fail to hatch (Lee et al. 2010).
PY  - 2020
JO  - microPublication Biology
ER  -