TY  - GEN
T1  - fs(1)A13041 is a 5′ UTR deletion of the essential gene small ovary in Drosophila
AU  - Hammond, Myles
AU  - Gomez, Jillian G.
AU  - Oliver, Brian
AU  - Kucera, Steve
AU  - Benner, Leif
DO  - 10.17912/micropub.biology.000246
UR  - http://beta.micropublication.org/journals/biology/micropub-biology-000246/
AB  - X-linked female sterile screens in Drosophila have led to a tremendous increase in our understanding of the genetic control of oogenesis (Gans et al. 1975; Mohler 1977; Komitopoulou et al. 1983). However, many of the loci in these screens have not been mapped to a single gene and therefore remain a rich resource for further elucidating the genetic control of female fertility. fs(1)A13041 is one such allele that is germline dependent and results in a degenerative ovary phenotype (Gans et al. 1975; Khipple and King 1976; Mulligan 1981; Wieschaus et al. 1981; Mulligan and Rasch 1985; Lamnissou and Gelti-Douka 1985). We were interested in determining the mutation that leads to sterility in fs(1)A13041 females. Previous recombination mapping had placed fs(1)A13041 at 19±2 cM on the X chromosome (Gans, Audit, and Masson 1975; Khipple and King 1976). We confirmed the previous mapping interval by meiotically mapping fs(1)A13041 to the right of crossveinless (12 cM) and to the left of singed (22 cM). We began complementation tests for female sterility with known deficiencies tiling the crossveinless and singed region and placed the lesion within a roughly 235 kb region (Figure 1A, non-complementing Df(1)BSC276, BSC285, BSC286, BSC297, BSC351, BSC535, and sov) (Parks et al. 2004; Cook et al. 2012). Two duplications within this narrow region rescued fs(1)A13041 sterility and thus further narrowed down the possible location of the causal mutation (Figure 1A, Dp(1;3)DC486 and Dp(1;3)DC026) (Venken et al. 2010). The mapping results were somewhat ambiguous within this narrow region (discussed below). However, the smallest non-complementing deficiency, Df(1)sov, contains only the protein coding gene small ovary (sov) and non-coding RNA gene CR43496. We therefore decided to complementation test fs(1)A13041 with known alleles of sov. Flies homozygous for hypomorphic alleles of sov show a similar female sterility phenotype to flies bearing fs(1)A13041 while amorphic sov alleles are embryonic lethal (Wayne et al. 1995; Jankovics et al. 2018; Benner et al. 2019). We found that amorphic alleles sovEA42 and sovML150 failed to complement fs(1)A13041 female sterility while the hypomorphic sov2 complemented fs(1)A13041 sterility. Collectively this indicates that fs(1)A13041 is a sov allele (sovA1304-1).
PY  - 2020
JO  - microPublication Biology
ER  -