TY  - GEN
T1  - A partial T-DNA insertion near KNAT1 results in lobed Arabidopsis thaliana leaves
AU  - Moulton, Karah
AU  - Diaz, Stephanie
AU  - Strother, Ashley
AU  - Hancock, C. Nathan
DO  - 10.17912/micropub.biology.000253
UR  - http://beta.micropublication.org/journals/biology/micropub-biology-000253/
AB  - T-DNA tagging is a method to generate mutations in plants by random insertion. It is an important tool for the study of gene function in Arabidopsis because it allows you to see how the plant responds when expression of a specific protein is altered. T-DNAs carrying promoter elements that can cause transcriptional activation, called activation tags, have previously been shown to be effective at identifying novel genes (Tani et al. 2004). We attempted to develop a gene silencing T-DNA, by modifying the existing microRNA-induced gene silencing (MIGS) platform (Han et al. 2015). In this platform, the transgene consists of a promoter driving expression of a miR173 target sequence directly adjacent to the gene sequence to be silenced (Zhang 2014). Expression produces an mRNA which is bound by the complementary, naturally occurring Arabidopsis microRNA, miR173. This induces the production of small interfering RNAs (siRNAs) which target homologous transcripts for degradation (Zhang 2014).
PY  - 2020
JO  - microPublication Biology
ER  -