TY  - GEN
T1  - Kavain suppresses human Aβ-induced paralysis in C. elegans
AU  - Chamoli, Manish
AU  - Chinta, Shankar J
AU  - Andersen, Julie K
AU  - Lithgow, Gordon J
DO  - 10.17912/micropub.biology.000254
UR  - http://beta.micropublication.org/journals/biology/micropub-biology-000254/
AB  - Kavain belongs to a group of lactone-based compounds collectively known as kavalactones, present in the pepper plant kava (P. methysticum). Kavalactones have been shown to possess diverse biological activities including sedation and anxiolysis (Ooi et al., 2018). Kavain in particular has been demonstrated to show potent anti-inflammatory properties in various in vitro and animal models (Guo et al., 2018; Singh et al., 2018; Tang and Amar, 2016; Yuan et al., 2011). A study in C. elegans reported that kavain increases lifespan by inhibiting advance glycation end-products (AGEs), which are known to suppress lifespan (Chaudhuri et al., 2016; Upadhyay et al., 2014). Another study reported that kavain increases acetylcholine (ACh) transmission at the neuromuscular junction (Kautu et al., 2017). Since loss in ACh transmission and increased formation of AGEs are closely linked to Aβ-pathology, we hypothesized that kavain may protect against Aβ-induced toxicity (Kar et al., 2004; Li et al., 2013). We tested kavain in the C. elegans GMC101 strain that over-expresses human Aβ in body wall muscle cells (McColl et al., 2012). Kavain at a concentration of 40 and 80 μM was shown to increase lifespan, thus we decided to use a dose between these ranges (Upadhyay et al., 2014). We observed GMC101 animals fed 50 μM kavain showed significantly less paralysis when shifted to the higher permissive temperature (25o C). The result shows that kavain suppresses Aβ-induced proteotoxicity.
PY  - 2020
JO  - microPublication Biology
ER  -