TY  - GEN
T1  - A null mutation of C. elegans vwa-8
AU  - Zhu, Ming
AU  - Chisholm, Andrew D
AU  - Jin, Yishi
DO  - 10.17912/micropub.biology.000263
UR  - http://beta.micropublication.org/journals/biology/micropub-biology-000263/
AB  - VWA8 proteins, named for von Willebrand factor A (VWA) domain containing 8, are conserved from worm to mammals (Whittaker & Hynes, 2002). In human, two SNPs (rs9566845 and rs9566867) in vwa8 are found to be associated with bipolar disorder with comorbid migraine (Oedegaard et al., 2010). Another SNP (rs9532931) is tentatively associated with a specific sub-group of autism patients (Anney et al., 2010). The C. elegans VWA-8 long isoform shares 38% and 55% amino acid sequence identity and similarity, respectively, with human VWA8 long isoform. We showed that endogenous VWA-8 is expressed in mitochondria of somatic tissues, except neurons (Zhu, Chisholm & Jin, 2020). To determine the function of C. elegans vwa-8, we generated a null allele vwa-8(ju1659) by CRISPR-Cas9. vwa-8(ju1659) mutants are homozygous viable, and indistinguishable from wild type in gross phenotypes such as body size, brood size, growth rate and movement.
PY  - 2020
JO  - microPublication Biology
ER  -