TY  - GEN
T1  - Pharyngeal pumping phenotypes of common fluorescently-tagged C. elegans strains
AU  - Bauer, Rosemary
AU  - Golden, Andy
DO  - 10.17912/micropub.biology.000267
UR  - http://beta.micropublication.org/journals/biology/micropub-biology-000267/
AB  - Genetic balancer strains are frequently used to maintain populations of C. elegans mutants that would otherwise die as homozygotes. Additionally, fluorescently-marked strains can be used to identify cross-progeny for use in assays where heterozygotes are of particular interest. In our case, we wanted to utilize a fluorescently-marked but otherwise wildtype strain to obtain marked cross-progeny from a mating. Our mutant of interest displayed altered pharyngeal pumping, so we wanted to assess heterozygotes in the same assay and establish the recessive nature of the phenotype. We happened to have the TY4236 strain growing in the lab, which not only expresses bright pharyngeal and gut GFP from the mIs10 insertion but also contains him-8(e1489), making the experiment quite simple. Due to the bright GFP signal in the pharynx we decided to assess the pharyngeal pumping of the strain itself before using it as a marker in an experiment that heavily implicates the pharynx.
PY  - 2020
JO  - microPublication Biology
ER  -