TY  - GEN
T1  - Generating reliable hypomorphic phenocopies by RNAi using long dsRNA as diluent
AU  - Bekas, Kimberly N
AU  - Phillips, Bryan T
DO  - 10.17912/micropub.biology.000269
UR  - http://beta.micropublication.org/journals/biology/micropub-biology-000269/
AB  - In C. elegans, RNAi is performed by delivering double-stranded RNA (dsRNA) to the worm via feeding (Timmons and Fire 1998), injection (Fire et al. 1998) or soaking (Tabara, Grishok, and Mello 1998). In feeding RNAi, sequence of a target gene is placed between two oppositely oriented promoter sequences active in E. coli (e.g. T7 in the pL4440 vector (Timmons and Fire 1998)). Subsequent T7 polymerase expression in E. coli provides sense and anti-sense transcription of the target gene sequence, generating dsRNA. The bacteria producing the dsRNA is consumed by the worm and the dsRNA distributed to C. elegans cells (Timmons and Fire 1998), resulting in efficient target gene knockdown.
PY  - 2020
JO  - microPublication Biology
ER  -