TY  - GEN
T1  - Gel-free genotyping of deletion alleles in Caenorhabditis elegans with real-time PCR
AU  - Wimberly, Keon
AU  - Choe, Keith P
DO  - 10.17912/micropub.biology.000274
UR  - http://beta.micropublication.org/journals/biology/micropub-biology-000274/
AB  - C. elegans is a powerful model system for studying the genetic basis of biological processes. Identifying relevant genes and organizing them into genetic pathways often involve analyzing phenotypes when mutations for two or more genes are combined in the same strain. This requires crossing and genotyping resulting individual F2 lineages. In the simplest case, mutant alleles cause robust phenotypes that can be observed with a microscope. Alleles without easily observable phenotypes can be followed by including closely linked alleles with obvious phenotypes (Wang and Sherwood 2011; Fay 2013). In some cases, neither of these scenarios are possible, which leaves the mutated allele as the only factor for selection.
PY  - 2020
JO  - microPublication Biology
ER  -