TY  - GEN
T1  - Single-cell resolution long-term luciferase imaging in cultivated Drosophila brains
AU  - Schubert, Frank K.
AU  - Helfrich-Förster, Charlotte
AU  - Rieger, Dirk
DO  - 10.17912/micropub.biology.000280
UR  - http://beta.micropublication.org/journals/biology/micropub-biology-000280/
AB  - In Drosophila melanogaster, approximately 150 so-called clock neurons express the molecular components of the circadian clock mechanism. The activity of these neurons enables the fly to anticipate and track cyclic changes in their environment and to respond to them accordingly. The core of the circadian clock is built by two interlocked transcriptional feedback loops. In the first loop, the transcription factors CLOCK (CLK) and CYCLE (CYC) initiate the expression of the core clock proteins PERIOD (PER) and TIMELESS (TIM), which are in turn repressing the activity of CLK and CYC as transcriptional activators, resulting in a cyclic accumulation of PER and TIM (reviewed in Hermann-Luibl and Helfrich-Förster, 2015). In light:dark cycles, the intracellular photoreceptor Cryptochrome (CRY) mediates light information to the core clock and resets the molecular mechanism, thereby synchronizing the clock every day at sunrise to the 24h oscillation (reviewed by Helfrich-Förster, 2020). However, in constant darkness, the oscillation of the clock proteins PER and TIM cycles with the endogenous period, which is close to, but not exactly 24hrs.
PY  - 2020
JO  - microPublication Biology
ER  -