TY  - GEN
T1  - The teratogenic effect of Triclosan on embryogenesis is attenuated by Tween 20 in Caenorhabditis elegans
AU  - Park, Youngyong
AU  - Gaddy, Matthew A.
AU  - Alfhili, Mohammad A.
AU  - Lee, Myon Hee
DO  - 10.17912/micropub.biology.000282
UR  - http://beta.micropublication.org/journals/biology/micropub-biology-000282/
AB  - Triclosan (TCS) is a chlorinated, phenolic antimicrobial (Fig. 1A) widely used in personal care products, such as antiseptics and disinfectants, and as an additive in cosmetics, household cleaners, plastics, paints, and textiles, among others (Alfhili and Lee 2019). Despite restricted commercial use in the USA, TCS is still detected at very high rate in human samples (Weatherly and Gosse 2017), possibly because of increased use in building materials, or older polypropylene copolymers (PPCs). TCS has raised concerns regarding its health risks and environmental impact (Alfhili et al.. 2019; Weatherly and Gosse 2017; Yueh and Tukey 2016). In terms of risk assessment, C. elegans has been a successful animal model for toxicological profiling as it allows for monitoring of vital physiological endpoints such as body length, locomotion, development, brood size, and survival (Meyer and Williams 2014). We have previously shown that TCS disrupts SKN-1/Nrf2-mediated oxidative stress response in C. elegans larvae (Yoon et al.. 2017). Here, we examine the hatching rate of C. elegans wild-type (N2) embryos in response to acute TCS exposure (see Methods). As shown in Fig. 1B-1D, TCS caused pronounced shrinkage of the inner cell mass of embryos in a dose-dependent fashion. As the inner cell mass shrinks, we speculate that TCS may disturb the osmotic regulation of the developing embryo. Of note, we have recently reported that non-ionic surfactants antagonize the toxicity of phenolic endocrine-disrupting chemicals in C. elegans larvae (Alfhili et al.. 2018). Likewise, the non-ionic surfactant polysorbate 20 (also known as Tween 20; Tw20), significantly ameliorated TCS-induced mortality and restored hatching to physiological rates (Fig. 1B and 1E). Since hydrophobic substances may be emulsified in micelles formed by non-ionic surfactants, we suggest that Tw20 may inhibit TCS-induced embryonic mortality by micellar solubilization.
PY  - 2020
JO  - microPublication Biology
ER  -