TY  - GEN
T1  - Expression of a FRET-based ATP Biosensor in the C. elegans Intestine
AU  - Soto, Jose
AU  - Rivera, Madeline
AU  - Broitman-Maduro, Gina
AU  - Maduro, Morris F
DO  - 10.17912/micropub.biology.000284
UR  - http://beta.micropublication.org/journals/biology/micropub-biology-000284/
AB  - The C. elegans intestine is a major site of the generation and storage of chemical energy. Adenosine triphosphate (ATP) is a major molecule involved in energy transfer, hence regulation of intracellular ATP levels is a critical aspect of metabolism. Recently, ATP sensor proteins have been used to indirectly measure ATP levels in living cells (Zhang et al., 2018). These sensors are derived by inserting the ε subunit of the bacterial F0F1-ATP synthase between two fluorescent protein domains, allowing detection of protein bound to ATP by Förster Resonance Energy Transfer, or FRET (Imamura et al., 2009). Others have recently demonstrated use of FRET-based ATP sensors in the C. elegans pharynx and muscle (Tsuyama et al., 2013; Wang et al., 2019). Here we test the intestinal expression of a similar FRET-based ATP sensor derived from the green/red fluorochrome pair Clover/mApple (Figure 1A) (Mendelsohn et al., 2018). When the protein is bound to ATP, the two fluorochromes are brought into proximity, permitting emission from Clover to excite mApple (Imamura et al., 2009; Mendelsohn et al., 2018).
PY  - 2020
JO  - microPublication Biology
ER  -