ceh-84, an unusual homeobox gene

Reilly, Molly; Hobert, Oliver

ceh-84, an unusual homeobox gene

Molly Reilly1 and Oliver Hobert1,2

1Columbia University, Department of Biological Sciences
2Howard Hughes Medical Institute

Correspondence to: Oliver Hobert (or38@columbia.edu)

Figure 1: Location, features and expression of the ceh-84 homeobox gene. A: Parts of the previously described C. elegans-specific gene cluster on chromosome II are shown. The homeobox genes ceh-81 through ceh-85 are outlined and labelled in orange and are surrounded by MATH and BTB type genes in green. All other genes and non-coding RNAs are colored gray. B: ceh-84 and ceh-85 genomic region with numerous pseudogenes highlighted in gray, protein coding genes in blue, and non-coding RNAs in black. MATH and BTB type genes labelled in green, homeobox genes labelled in orange, all others in black. C: Further zoom in on ceh-84 and ceh-85 loci showing schematic of ceh-84 reporter with insertion of GFP sequence before the stop codon. Pseudogenes are colored in gray, protein coding genes belonging to the homeobox family in orange, protein coding genes belonging to the MATH and BTB type in green, all others in black. D: CEH-84 contains two unusual, short homeodomains. Orange indicates the sequence match to the SSF46689 superfamily domain, a homeodomain signature automatically generated by http://supfam.org. The numbering below indicates the position of canonical, usually 60 aa long homeodomains and their predicted alpha helices (gray bars), with helix 3 binding in the major groove of DNA (Bürglin and Affolter, 2016). This numbering is anchored with the highly conserved WF residues (underlined). E: Images of CEH-84 protein fusion reporter, PHX2811 (allele syb2811), generated by insertion of gfp sequence by CRISPR/Cas9. On the left, embryonic GFP expression looks diffuse and non-nuclear. On the right, no detectable GFP in L4 hermaphrodites. Solid white lines outline the worm structures, dashed lines highlight the intestinal GFP autofluorescence, and the white V marks the vulva. Using a ~2kb promoter fusion, postembryonic intestinal expression was observed by McKay et al., 2003 and weak embryonic expression was noted by Hench at al., 2015.

Description

We have previously described the expression patterns of all but one of the 102 homeobox genes present in the C. elegans genome, using either fosmid-based reporter transgenes or CRISPR/Cas9-engineered gfp reporter alleles (Reilly et al., 2020). The last remaining homeobox gene for which we were initially not able to generate a reporter reagent is ceh-84 (Reilly et al., 2020). As previously recognized by Thomas Bürglin (Hench et al., 2015), ceh-84 codes for an unusual homeodomain protein with two divergent homeodomains (Fig.1D). It likely is a tandem duplicate of the ceh-85 gene, which has been classified as a pseudogene (Fig.1B). ceh-84 has no recognizable homologs in other Caenorhabditis species (Hench et al., 2015). Together with its duplicate, ceh-85, it localizes to a large cluster of extensively duplicated genes on chromosome II, described by James Thomas (Thomas, 2006). Most of the duplicated genes code for MATH and BTB type proteins (Fig.1A). The entire cluster seems to be C. elegans-specific (Thomas, 2006) and, apart from a number of pseudogenes, it also contains the C. elegans-specific ceh-81, ceh-82 and ceh-83 homeobox genes (Fig.1A).

We designed a gfp reporter allele of ceh-84 that was generated by CRISPR/Cas9 genome engineering. We observed no expression of the protein in any cells in larvae and adults (Fig.1E). In embryos we observe very dim, diffuse GFP signals that make it unlikely that any functional protein is present in the nucleus at any stage. The absence of obvious CEH-84 in the mature nervous system is consistent with our previously reported observation that nematode or C. elegans-specific homeobox genes make no significant contribution to the combinatorial code of homeobox gene expression in the nervous system (Reilly et al., 2020).

Reagents

PHX2811 – ceh-84(syb2811[ceh-84::GFP]). Generated and provided by Sunybiotech.

Methods

Request a detailed protocol

For imaging, worms were anesthetized using 100mM of sodium azide (NaN₃) and mounted on 5% agarose pad on glass slides. Images were acquired at 40x using a confocal laser scanning microscope (Zeiss LSM880) and processed using the ImageJ software. Representative maximum intensity projections are shown for GFP channel as gray scale and gamma and histogram were adjusted for visibility.

References

Bürglin, T.R., and Affolter, M. (2016). Homeodomain proteins: an update. Chromosoma 125, 497-521

PubMed

Hench, J., Henriksson, J., Abou-Zied, A.M., Luppert, M., Dethlefsen, J., Mukherjee, K., Tong, Y.G., Tang, L., Gangishetti, U., Baillie, D.L., et al. (2015). The Homeobox Genes of Caenorhabditis elegans and Insights into Their Spatio-Temporal Expression Dynamics during Embryogenesis. PLoS One 10, e0126947.

PubMed

McKay, S.J., Johnsen, R., Khattra, J., Asano, J., Baillie, D.L., Chan, S., Dube, N., Fang, L., Goszczynski, B., Ha, E., et al. (2003). Gene expression profiling of cells, tissues, and developmental stages of the nematode C. elegans. Cold Spring Harb Symp Quant Biol 68, 159-169.

PubMed

Reilly, M.B., Cros, C., Varol, E., Yemini, E., and Hobert, O. (2020). Unique homeobox codes delineate all the neuron classes of C. elegans. Nature 584, 595-601.

PubMed

Thomas, J.H. (2006). Analysis of homologous gene clusters in Caenorhabditis elegans reveals striking regional cluster domains. Genetics 172, 127-143.

PubMed

Funding

This work was funded by a predoctoral fellowship to Molly Reilly (F31 NS105398), by NIH R21 NS106843, and by the Howard Hughes Medical Institute

Author Contributions

Molly Reilly: Conceptualization, Investigation, Formal analysis, Writing - review and editing
Oliver Hobert: Conceptualization, Project administration, Supervision, Funding acquisition, Writing - original draft.

Reviewed By

Anonymous

History

Received: November 11, 2020
Revision received: December 18, 2020
Accepted: December 1, 2020
Published: December 1, 2020

Copyright

© 2020 by the authors. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International (CC BY 4.0) License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Citation

Reilly, M; Hobert, O (2020). ceh-84, an unusual homeobox gene. microPublication Biology. 10.17912/micropub.biology.000340.
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