Description
In C. elegans, the reuptake of serotonin (5-HT) is facilitated by mod-5, which encodes a 5-HT transporter that is orthologous to a human 5-HT transporter (SLC6A4). mod-5 has been shown to effect both feeding and locomotion in C. elegans (Ranganathan et al., 2001; Jafarau et al. 2011). We obtained and analyzed EPG data using a microfluidic device (NemaMetrix) for mod-5 null mutant strains, mod-5(n3314) (A, Exp 1 n=15; Exp 2 n=32) and mod-5(n822), (B, Exp 1 n=17; Exp 2 n=27) and N2 control worms (A, n=16 and 31; B, n=18 and 27 for Exp 1 and 2, respectively) in M9 saline buffer (2-minute recording duration). Mutations to the C. elegans serotonin reuptake transporter, mod-5, lead to an accumulation of serotonin at the synaptic cleft, which results in a significant increase in baseline pharyngeal pumping frequency in three out of four experiments (A, N2=0.10 ± 0.04 and 0.09 ± 0.03 Hz ; mod-5(n3314)=1.41 ± 0.44 and 2.26 ± 0.13 Hz; B, N2=0.98± 0.42 and 0.44 ± 0.08; mod-5(n822)=1.64 ± 0.33 and 3.76 ± 0.19 Hz; **p<0.005, ***p<0.0001, 2-tailed students t-test).
References
Funding
Oregon Nanoscience and Microtechnologies Institute (ONAMI) and NemaMetrix, Inc.
Reviewed By
Veena PrahladHistory
Received: December 12, 2016Accepted: January 27, 2017
Published: February 8, 2017
Copyright
© 2017 by the authors. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International (CC BY 4.0) License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.Citation
Hiebert, T; Chicas-Cruz, A; McCormick, K (2017). Basal pharyngeal pumping elevated in C. elegans mod-5 mutants. microPublication Biology. 10.17912/W2H59W.Download: RIS BibTeX