Description

Recently, the GAL4-UAS system (cGAL) has been adapted for use in C. elegans for control of gene expression across 15°C – 25°C (Wang et al., 2017). In order to create a desired gene expression pattern, one crosses a transgenic strain containing a driver construct with another strain containing an effector gene. Here we mapped several cGAL driver and effector integrations. We first crossed each of the cGAL driver and effector strains with N2 males, picked the heterozygous male progeny, crossed them with hermaphrodites of the mapping strain (DA438), picked L4 hermaphrodites with the corresponding transgenic marker of the driver or effector strain and scored the progeny in the next generation. The DA438 strain contains six recessive mutations, each of which locates on one of the six chromosomes and produces visible phenotypes (Bli on chromosome I, Rol on II, Vab on III, Unc on IV, Dpy on V, and Lon on X (Avery, 1993). F2 progeny with each of the six phenotypes were selected and examined for the presence or absence of the dominant marker associated with the transgene. In the cases where the dominant transgene marker is unlinked to the recessive phenotypic marker, about three quarters of the F2 progeny will have the dominant marker. If the two markers are linked, very few or no animals are expected to have the dominant transgenic marker. The following tables summarize the mapping results for each cGAL strain, stating the ratios of the F2 mutant progeny with and without the dominant transgenic marker.

Effectors

15xUAS::GFP for Cell Labeling

15xUAS::mKate2 for cell Labeling

15xUAS::hChR2(H134R)::YFP for neuronal activation

15xUAS::TeTx (Tetanus toxin light chain) for blocking synaptic transmission

15xUAS::HisCl1::SL2::GFP for neuronal inhibition

Drivers

Pharyngeal muscle driver, Pmyo-2

Heat shock driver, Phsp-16.41

Pan-neuronal driver, Prab-3

Intestinal driver, Pnlp-40

Body muscle driver, Pmyo-3