Howard Hughes Medical Institute, California Institute of Technology, Pasadena, California, USA
Description
Summary a new allele of pkd-2 was isolated in a behavioral genetic screen for male mating defects, and found to result in a substitution of Arginine for Glycine in the equivalent of human PKD2 alanine 615.
Article
The C. elegans ortholog of polcystin-2 is encoded by pkd-2 (Barr et al., 2001). From an EMS screen of a plg-1; him-5 strain for male mating defective mutants and a secondary behavioral screen for defects in discrete steps of male mating behavior, namely response to contact to hermaphrodites and vulval location (described in Schindelman et al., 2006), we identified a new allele of pkd-2 based on mapping and complementation. sy680fails to complement pkd-2(sy606) for defects in response to contact with hermaphrodite and vulval location. Here we report the sequence of this allele. PCR amplification and sequencing of pkd-2 exons indicated that there was a c–>t transition in the transcribed DNA strand (g–>a in the pkd-2 sense strand; Figure 1A). This change leads to an altered codon, a Glycine to Arginine substitution the PKD-2 protein. This position corresponds to A615 of the human protein (Figure 1B).
References
Funding
Supported by the Howard Hughes Medical Institute (047-101), with which PWS is an investigator.
Reviewed By
Bob O'HaganHistory
Received: September 13, 2017Accepted: September 28, 2017
Published: September 28, 2017
Copyright
© 2017 by the authors. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International (CC BY 4.0) License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.Citation
Whittaker, A; Schindelman, G; Gharib, S; Sternberg, PW (2017). sy680 is a novel allele of pkd-2. microPublication Biology. 10.17912/W2SW9M.Download: RIS BibTeX