Description
Stellaris smFISH probes targeting acdh-1, a short-chain acyl-CoA dehydrogenase, are shown in red (Cal Fluor 610). DAPI/blue marks embryonic nuclei, and PGL-1::GFP shows the corresponding location of P granules surrounding germ cell nuclei (arrowheads, green). During embryogenesis (A), acdh-1 expression begins in the E cells (arrow, red). Expression continues in the developing intestine, shown in red, throughout embryogenesis. Intestinal expression of acdh-1 persists through larval development in the L1 (B) and L2 (C) stages. These results extend previous findings from Arda et al., where acdh-1 was shown to be expressed in the adult intestine. scale = 20µ.
Reagents
The Stellaris acdh-1 smFISH probe contains 44 oligos labeled with Cal Fluor 610 (sequences available upon request). Staining was performed as described by Ji and van Oudenaarden (2012). In short, PGL-1::GFP embryos and worms were fixed with formaldehyde and ethanol. Hybridization was done in the dark at 37°C for four hours. Cal Fluor 610 coupled probes were designed with the Stellaris Probe Designer from Biosearch Technologies.
References
Funding
NIH-NIGMS[R01GM113933]
Reviewed By
Jim LissemoreHistory
Received: July 2, 2017Accepted: July 8, 2017
Published: July 20, 2017
Copyright
© 2017 by the authors. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International (CC BY 4.0) License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.Citation
Updike, D (2017). acdh-1 transcripts are expressed in the intestine. microPublication Biology. 10.17912/W2V365.Download: RIS BibTeX
