Description

We report tm4476 and tm4561 as novel alleles of C38D4.9 gene that is an ortholog of human METTL5 (methyltransferase like 5)1. Human METTL5 function has not been reported. The alleles were isolated from the comprehensive screening of gene deletions generated by TMP/UV2. In the screening, both the alleles were detected by nested PCR using the following primer sets, 5’-CCGCCTATATCATGGCGCTT-3’ and 5’-GCTGGTAGTTGCCACCGCAT-3’ for first round PCR and 5’-ACTATCGAAGCCGCGCTTCA-3’ and 5’-AACCCCAACTCCGTCCCATT-3’ for second round PCR. By Sanger sequencing, the 30 bp flanking sequences of the alleles tm4476 and tm4561 were identified as GTATGAGTTGGAGACTGTTTTAGGAGTTGA-(191 bp deletion)-GTACTCTTTAAAAGCGCACATCTTTCTGAA and TAATTGATATCGGATGTGGATGTGGAATGT-(309 bp deletion)-ATTTTCGGCTAAAAATCGTATTTGGGTAAT, respectively (Fig. 1). Based on the information about the splicing isoforms of C38D4.9 (WormBase3 WS259), the second exon of C38D4.9 is deleted in tm4476 and tm4561.